Frontiers Journals May 8 2025 A recent study published in Engineering presents a novel approach to analyzing the low-abundant N- glycoproteome in human blood plasma (HBP), which could significantly advance the discovery of plasma biomarkers. Protein glycosylation plays a crucial role in clinical diagnostics and biopharmaceuticals. However, current N- glycoproteomic methods face challenges such as incorrect identifications, difficulties in detecting rare and modified N- glycans, and insufficient