Chinese Academy of Sciences Mar 11 2025 Preclinical drug evaluation, especially for cardiotoxicity, is a critical stage in the drug development process. Traditional methods, often relying on in vitro assays or manual manipulation of animal models, are both inefficient and unable to fully mimic in vivo conditions. Zebrafish, with their transparent bodies, rapid development, and genetic similarities to humans, are emerging as a powerful model for high-throughput screening. However, the existing