Beijing Institute of Technology Oct 10 2025 Recent research has achieved significant advances in acoustofluidic technologies for efficient isolation and biomarker-specific detection of small extracellular vesicles (sEVs). Nevertheless, rapid and high-sensitivity analysis of low-volume clinical samples remains challenging, often requiring multi-step preprocessing and bulky instrumentation. By integrating sharp-edge microstructures with acoustically induced vortices, we enable size-selective