Nanjing University of Science and Technology Sep 16 2025 Optical diffraction tomography (ODT) has long been recognized for its potential in non-invasive, label-free imaging of live biological cells. However, a major challenge arises when ODT is applied to standard multi-well cell culture plates, a common tool use under realistic laboratory conditions for biological studies. The geometry of these cell culture plates restricts the range of oblique illumination angles, causing a loss of critical