Juntendo University Jun 19 2025 Three dimensional immunohistochemistry (3D-IHC) has transformed our ability to visualize the spatial arrangement of cells and molecules in intact tissues. However, traditional methods are often time-consuming and suffer from poor antibody penetration, which limits their effectiveness in deep tissues. This bottleneck has posed significant challenges in neuroscience, pathology, and biomedical imaging, where rapid and detailed mapping of large tissue volumes is